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KMID : 0545120190290020230
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 2 p.230 ~ p.234
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A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042
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Sun Yunlong
Niu Yali
He Bin
Ma Long
Li Ganghua
Tran Van-Tuan
Zeng Bin
Hu Zhihong
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Abstract
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Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplastmediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.
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KEYWORD
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Aspergillus oryzae, uridine/uracil auxotrophy, pyrithiamine, Agrobacterium tumefaciens-mediated transformation
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